Fluorescence observables#
Steady-state fluorescence measurements: the Stokes shift between absorption and emission, and the Perrin fluorescence-anisotropy equation.
Stokes shift: fluorescence is emitted at longer wavelength than it is absorbed
Stokes shift: fluorescence is emitted at longer wavelength than it is absorbed
Perrin equation: fluorescence anisotropy and molecular rotation
Perrin equation: fluorescence anisotropy and molecular rotation